Keywords
fungus
infection
inoculation
virulence
How to Cite
Abstract
Anthracnose, associated with the phytopathogenic genus Colletotrichum, is one of the most harmful mango diseases in the world, causing field and postharvest damage. The pathogen is hemibiotrophic and an asymptomatic endophyte, and some species show quiescence; the role of these biological states as a source of inoculum is still uncertain. The pathogenicity of 14 Colletotrichum endophyte-quiescent isolates obtained from previous studies and one isolate from symptomatic mango fruit were evaluated. Initially, the isolates were described morphometrically (colony) from monosporic cultures and compared for growth rate, inoculum density, conidia, and appressoria, finding statistical differences for growth rate, inoculum density, and conidia size. The pathogenicity of the morphotypes on mango leaves and branches was evaluated with spray inoculations on two-year-old trees under dry tropical conditions; symptoms were described, and biological variables (number, diameter, and growth rate of lesions) were evaluated. In leaves, all morphotypes induced lesions but there were differences in growth rate; in branches, highly significant differences were found between morphotypes in the number and diameter of lesions and differences in lesion growth rate; however, there were morphotypes that did not induce lesions. Colletotrichum morphotypes have high morphological and biological variability, and endophytic isolates are potentially infective on mango leaves and branches.References
Akem, C. (2006). Mango Anthracnose Disease: Present Status and Future Research Priorities. Plant Pathology Journal, 5(3), 266-273. https://dx.doi.org/10.3923/ppj.2006.266.273.
Auyong, A., Ford, R. y Taylor, P. (2015). The Role of Cutinase and its Impact on Pathogenicity of Colletotrichum truncatum. Journal of Plant Pathology & Mycrobiology, 6(3), 1-11. http://dx.doi.org/10.4172/2157-7471.1000259.
Baquero, M., Peres, N. y Arauz, L. (2013). Presencia de Colletotrichum acutatum y Colletotrichum gloeosporioides en helecho hoja de cuero, limón criollo, papaya, carambola y mango en Costa Rica y Florida (Estados Unidos). Agronomía Costarricense, 37(1), 23-38. https://doi.org/10.15517/rac.v37i1.10710.
Barimani, M., Pethybridge, S., Vaghefi, N., Hay, F. y Taylor, P. (2013). A new anthracnose disease of pyrethrum caused by Colletotrichum tanaceti sp. nov. Plant Pathology, 62(6), 1248-1257. https://doi.org/10.1111/ppa.12054.
Bersisa, H., Dejene, M. y Derso, E. (2018). Isolation, Identification and Characterization of Colletotrichum Kahawae from Infected Green Coffee Berry in Arsi, Southeastern Etiopia. Journal of Biology, Agriculture and Healthcare, 8(21), 41-49.
Calo, L., García, I., Gotor, C. y Romero, L. (2006). Leaf hairs influence phytopathogenic fungus infection and confer an increased resistance when expressing a Trichoderma α-1,3-glucanase. Journal of Experimental Botany, 57(14), 3911-3920. https://doi.org/10.1093/jxb/erl155.
Carroll, G. (1998). Fungal Endophytes in Stems and Leaves: From Latent Pathogen to Mutualistic Symbiont. Ecology, 69(1), 2-9. https://doi.org/10.2307/1943154.
Damm, U., Cannon, P., Woudenberg, J. y Crous, P. (2012). The Colletotrichum acutatum species complex. Studies in Mycology, 73(1), 37-113. https://doi.org/10.3114/sim0010.
Dangond, F. (2018). Aislamientos endofíticos de Colletotrichum spp. en hojas de mango (Mangifera indica L.) cultivar Azúcar, en el municipio de Ciénaga (Magdalena, Colombia) [Tesis de pregrado, Universidad del Magdalena]. Repositorio de la Universidad del Magdalena. http://repositorio.unimagdalena.edu.co/handle/123456789/3795.
De Silva, D., Crous, P., Ades, P., Hyde, K. y Tylor, P. (2017). Life styles of Colletotrichum species and implications for plant biosecurity. Fungal Biology Reviews, 31(3), 155-168. https://doi.org/10.1016/j.fbr.2017.05.00.
De Silva, D., Groenewald, J., Crous, P., Ades, P., Nasruddin, A., Mongkolporn, O. y Taylor, P. (2019). Identification, prevalence and pathogenicity of Colletotrichum species causing anthracnose of Capsicum annuum in Asia. IMA Fungus, 10(8), 1-32. https://doi.org/10.1186/s43008-019-0001-y.
Domínguez, I., Mohail, S. y Pino, H. (2014). Análisis morfométrico del género Colletotrichum en palma aceitera. Agricultura Andina, 20, 80-96.
Gañán, L., Álvarez, E. y Castaño, J. (2015). Identificación genética de aislamientos de Collectotrichum spp. causantes de antracnosis en frutos de aguacate, banano, mango y tomate de árbol. Revista de la Académica Colombiana de Ciencias Exactas, Físicas y Naturales, 39(152), 339-347. https://doi.org/10.18257/raccefyn.192.
Granados, G. y Guevara, Y. (2010). Caracterización biológica y molecular de aislamientos de Colletotrichum spp., obtenidos de frutales de clima frío moderado con síntomas de antracnosis [Tesis de pregrado, Pontificia Universidad Javeriana de Colombia]. Repositorio de la Universidad Javeriana. https://repository.javeriana.edu.co/handle/10554/8474.
Hardoim, P., Van Overbeek, L., Berg, G., Pirttilä, A., Compant, S., Campisano, A., Döring, M. y Sessitsch, A. (2015). The Hidden World within Plants: Ecological and Evolutionary Considerations for Defining Functioning of Microbial Endophytes. Microbiology and Molecular Biology Reviews, 79(3), 293-320. https://doi.org/10.1128/mmbr.00050-14.
Harp, T., Pernezny, K., Ivey, M., Miller, S., Kuhn, P. y Datnoff, L. (2008). The etiology of recent pepper anthracnose outbreaks in Florida. Crop Protection, 27(10), 1380-1384. https://doi.org/10.1016/j.cropro.2008.05.006.
Hernández, P., Aranguren, M., Reig, C., Fernández, D., Mesejo, C., Martínez, A., Galán, V. y Agustí, M. (2011). Phenological growth stages of mango (Mangifera indica L.) according to the BBCH scale. Scientia Horticulturae, 130(3), 536-540. https://doi.org/10.1016/j.scienta.2011.07.027.
Hyde, K. y Soytong, K. (2008). The fungal endophyte dilemma. Fungal Diversity, 33, 163-173.
Kamle, M. y Kumar, P. (2016). Colletotrichum gloeosporioides: Pathogen of Anthracnose Disease in Mango (Mangifera indica L.). En P. Kumar, V. Gupta, A. Tiwari y M. Kamle (Eds.), Current Trends in Plant Disease Diagnostics and Management Practices (pp. 1-440). Springer.
Kamle, M., Kumar, P., Gupta, V., Tiwari, A., Misra, A. y Pandey, B. (2013). Identification and phylogenetic correlation among Colletotrichum gloeosporioides pathogen of anthracnose for mango. Biocatalysis and Agricultural Biotechnology, 2(3), 285-287. https://doi.org/10.1016/j.bcab.2013.04.001.
Majumdar, N. y Mandal, N. (2018). Effect of pH on Mycelial Growth and Sporulation of Postharvest Pathogen Colletotrichum gloeosporioides (Penz.) Penz & Sacc. and Pestalotiopsis mangiferae (Henn.) Steyaert. International Journal of Bio-resource and Stress Management, 9(1), 416-420. https://doi.org/10.23910/ijbsm/2018.9.3.1874
Oliveira, R., Moral, J., Bouhmidi, K. y Trapero, A. (2005). Caracterización morfológica y cultural de aislados de Colletotrichum spp. causantes de la antracnosis del olivo. Boletín de Sanidad Vegetal Plagas, 31, 531-548.
Páez, A. (2003). Tecnologías sostenibles para el manejo de la antracnosis en papaya y mango (Boletín Técnico N.º 8). Corpoica.
Páez, A. (2020). Biología y manejo de estados quiescentes de Colletotrichum spp. en mango cultivar Azúcar, en el departamento del Magdalena, Colombia [Tesis de doctorado, Universidad Nacional de Colombia]. Repositorio de la Universidad Nacional de Colombia. https://repositorio.unal.edu.co/bitstream/handle/unal/82503/5056184.2020.pdf?sequence=2&isAllowed=y.
Pavitra, R. y Singh, R. (2017). Anthracnose of mango incited by Colletotrichum gloeosporioides: A Comprehensive Review. International Journal of Pure & Applied Bioscience, 5(1), 48-56. http://dx.doi.org/10.18782/2320-7051.2478.
Perfect, S., Hughes, H., O’Connell, R. y Green, J. (1999). Colletotrichum: A Model Genus for Studies on Pathology and Fungal-Plant Interactions. Fungal Genetics and Biology, 27(2-3), 186-198. https://doi.org/10.1006/fgbi.1999.1143.
Photita, W., Lumyong, S., Lumyong, P., McKenzie, E. y Hyde, K. (2004). Are some endophytes of Musa acuminata latent pathogens? Fungal Diversity, 16, 131-140.
Prihastuti, H., Cai, L., Chen, H., McKenzie, E. y Hyde, K. (2009). Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity, 39, 89-109.
Prusky, D. y Lichter, A. (2007). Activation of quiescent infections by postharvest pathogens during transition from the biotrophic to the necrotrophic stage. Microbiology Letters, 268(1), 1-8. https://doi.org/10.1111/j.1574-6968.2006.00603.x.
Prusky, D., Alkan, N., Mengiste, T. y Fluhr, R. (2013). Quiescent and Necrotrophic Lifestyle Choice During Postharvest Disease Development. Annual Review of Phytopathology, 51, 155-176. https://doi.org/10.1146/annurev-phyto-082712-102349.
Quintero, A., Dangond, F. y Páez, A. (2019). Aislamientos endofíticos de Colletotrichum spp. a partir de hojas y ramas de mango (Mangifera indica L.) cultivar Azúcar en el municipio de Ciénaga, Magdalena, Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 43(166), 65-77. http://dx.doi.org/10.18257/raccefyn.788.
Rodríguez, R., White, J., Arnold, A. y Redman, R. (2009). Fungal endophytes: diversity and functional roles. New Phytologist, 182(2), 314-330. https://doi.org/10.1111/j.1469-8137.2009.02773.x.
Rojo, I., Álvarez, B., García, R., León, J., Sañudo, A. y Allende, R. (2017). Situación actual de Colletotrichum spp. en México: Taxonomía, caracterización, patogénesis y control. Revista Mexicana de Fitopatología, 35(3), 549-570. https://doi.org/10.18781/r.mex.fit.1703-9.
Sánchez, R., Sánchez, B., Monserrat, Y., Ulloa, A., Armendáriz, B., García, M. y Macías, M. (2013). Hongos endófitos: fuente potencial de metabolitos secundarios bioactivos con utilidad en agricultura y medicina. Revista Especializada en Ciencias Químico-Biológicas, 16(2), 132-146. https://doi.org/10.1016/S1405-888X(13)72084-9.
Sangeetha, C. y Rawal, R. (2008). Nutritional Studies of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. The Incitant of Mango Anthracnose. World Journal of Agricultural Sciences, 4(6), 717-720.
Scot, N. (2008). Mango anthracnose (Colletotrichum gloeosporioides). Plant Disease, 1-7.
Serrano, M., Coluccia, F., Torres, M., L’Haridon, F. y Métraux, J. (2014). The cuticle and plant defense to pathogens. Frontiers in Plant Science, 5, 1-8. https://dx.doi.org/10.3389%2Ffpls.2014.00274.
Sharma, G., Maymon, M. y Freeman, S. (2017). Epidemiology, pathology and identification of Colletotrichum including a novel species associated with avocado (Persea americana) Anthracnose in Israel. Scientific Reports, 7, 1-16. https://doi.org/10.1038/s41598-017-15946-w.
Stone, J., Bacon, C. y White, J. (2000). An overview of endophytic microbes: Endophytism defined. En C. Bacon y J. White (Eds.), Microbial endophytes (pp. 1-391). Elsevier.
Valdés, L., Calero, D., Carballo, M., Capote, M., González, I., Álvarez, J. y Rohde, W. (2017). Caracterización morfológica, cultural y patogénica de aislados de Colletotrichum sp. produciendo antracnosis en mango (Mangifera indica L.). La Granja: Revista de Ciencias Naturales, 26(2), 38-51.
Vieira, W., Michereff, S., de Morais, M., Hyde, K. y Câmara, M. (2014). Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal Diversity, 67, 181-202. https://doi.org/10.1007/s13225-014-0293-6.
Whitelaw, W., Curtin, S., Huang, R., Steel, C., Blanchard, C. y Roffey, P. (2007). Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia. Plant Pathology, 56(3), 448-463. https://doi.org/10.1111/j.1365-3059.2007.01569.x.
Wilson, D. (1995). Endophyte: The Evolution of a Term, and Classification of Its Use and Definition. Oikos, 73(2), 274-276. https://doi.org/10.2307/3545919.
Abdo, M.S.A. and Da Silva, C.J. (2000). Nutrient Stock in the Aquatic Macrophytes Eichhornia crassipes and Pistia stratiotes in the Pantanal-Brazil. In of the German-Brazilian Workshop on Neotropical Ecosystems. Hamburg, 3, 8, 875-880.
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