Intropica
Molecular diagnosis in Giardiasis: its potentialities
PDF (Español (España))

Keywords

Giardia intestinalis
diarrhea
genotyping
RFLP
phylogeography

How to Cite

Villalba Vizcaíno, V. (2012). Molecular diagnosis in Giardiasis: its potentialities. Intropica, 7(1), 91–96. Retrieved from https://revistas.unimagdalena.edu.co/index.php/intropica/article/view/167

Abstract

Giardia intestinalis is a parasite of worldwide distribution, causing acute non-bacterial diarrhea. Parasite transmission is fecal-oral and it can get between humans, humans-animals and animals-animals, being waterborne transmission main, so it is generally considered that this disease is an important health problem.. This protozoan parasite has a high genetic variability, because it has a diverse range of host. This protozoan has eight genotypes identified, with a host-specific relationship; but some of them could be non-specific, which allows zoonotic transmission. There are some techniques conventionally used for the diagnosis of infection with G. intestinalis, however these have low sensitivity due to intermittent elimination of cysts; but to investigate this area, many new techniques are available, such as Polymerase Chain Reaction(PCR), Restriction fragment lenght polymorphism (RFLP), Sequencing. It’s not clear the role of pets in zoonotic transmission, genotype distribution in our environment and impact in health population. The aim of this paper is showing particular characteristics of G. intestinalis, health problems that this parasite represent to population, the molecular tools available and its potentiality to resolve some questions about this important protozoan.
PDF (Español (España))

References

Al-Mohammed, H. I. 2011. Genotypes of Giardia intestinalis clinical isolates of gastrointestinal symptomatic and asymptomatic Saudi children. Parasitology Research 108 (6):1375-81.

Botero, D. y M. Restrepo. 2012. 5a Edición. Parasitosis Humana. Editorial CIB. pp: 79-88.

Calderaro, A., C. Gorrini, S. Montecchini, S. Peruzzi, G. Piccolo, S. Rossi, F. Gargiulo, N. Manca, G. Dettori y C. Chezzi. 2010. Evaluation of a real-ime polymerase chain reaction assay for the laboratory diagnosis of giardiasis. Diagnostic Microbiology Infectious Disease 66 (3): 261-267.

Chakarova, B. 2010. Comparative evaluation of the diagnostic methods for detection of Giardia intestinalis in human fecal samples. Trakia Journal of Sciences 8: 174-179.

Cotton, J. A., J.K. Beatty, y A.G. Buret. 2011. Host parasite interactions and pathophysiology in Giardia infections. International Journal for Parasitology 41 (9): 925-933.

El-Badry, A. A., K.H. Al-Ali y A-R. S. Mahrous. 2010. Molecular identification & prevalence of Giardia lamblia & Cryptosporidium in duodenal aspirate in Al- Madinah. Journal of Medicine and Biomedical ciences 1 (2): 47-52.

El-Nahas, H.A., D.A. Salem, A.A. El-Henawy, H.I. El-Nimr, H.A. Abdel-Ghaffar, y A.M. El-Meadawy. 2013. Giardia diagnostic methods in human fecal samples: a comparative study. Cytometry part B (Clinical Cytometry) 84B: 44-49.

Elsafi, S.H., T.N. Al-Maqati, M.I. Hussein, A.A. Adam,M.M. Abu Hassan y E.M. Al Zahrani. 2013. Comparison of microscopy, rapid immunoassay and molecular techniques for the detection of Giardia lamblia and Cryptosporidium parvum. Parasitology Research. DOI 10.1007/s00436-013-3319-1.

Eligio-Garcia, L., A. Cortés-Campos, S. Cota-Guajardo, S. Gaxiola y E. Jimenez-Cardoso. 2008. Frequency of Giardia intestinalis assemblages isolated from dogs and humans in a community from Culiacan, Sinaloa, Mexico using betagiardin restriction gene. Veterinary Parasitology Journal 156 (3-4): 205-209.

Fernandes, L.N., P.P. de Souza, R.S. de Araujo, M.T. Razzolini, R.M.Soares, M.I. Sato, E.M. Hachich, S.A. Cutolo, G.R. Matte, y M.H. Matte. 2011. Detection of assemblages A and B of Giardia duodenalis in water and sewage from Sao Paulo state, Brazil. Journal of Water and Health 9 (2): 361-7.

Geurden, T., D. Berkvens, S. Casaert, J. Vercruysse y E. Claerebout. 2008. A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogs. Veterinary Parasitology Journal 157 (1-2): 14-20.

Ivanov, A.I. 2010. Giardia and giardiasis. Bulgarian Journal of Veterinary Medicine 13: 65-80.

Kohli, A., O.Y. Bushen, R.C. Pinkerton, E. Houpt, R.D. Newman, C.L. Sears, A.A. Lima y R.L. Guerrant. 2008. Giardia duodenalis assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children. Transactions of the Royal Society of Tropical Medicine and Hygiene 102 (7): 718-725.

Lebbad, M., I. Petersson, L. Karlsson, S. Botero-Kleiven, J.O. Andersson, B. Svenungsson y S.G. Svard. 2011. Multilocus genotyping of human Giardia isolates suggests limited zoonotic transmission and association between assemblage B and flatulence in children. PLoS Neglected Tropical Disease 5 (8):e1262. doi:10.1371/journal.pntd.0001262.

Lee, J. H., J. Lee, S.J. Park, T.S. Yong y U.W. Hwang. 2006. Detection and genotyping of Giardia intestinalis isolates using intergenic spacers(IGS)-based PCR. Korean Journal of Parasitology 44 (4): 343-353.

Lymbery, A.J. y R.C. Thompson. 2012. The molecular epidemiology of parasite infections: tools and applications. Molecular and Biochemical Parasitology Journal 181 (2): 102-116.

Mohammed Mahdy, A.K., J. Surin, K.L. Wan, A. Mohd-Adnan, M.S. Al-Mekhlafi y Y.A. Lim. 2009. Giardia intestinalis genotypes: Risk factors and correlation with clinical symptoms. Acta Tropica Journal 112 (1): 67-70.

Molina, N., B. Pezzani, M. Ciarmela, A. Orden, D. Rosa,M. Apezteguia, J. Basualdo y M. Minvielle. 2011. Intestinal parasites and genotypes of Giardia intestinalis in school children from Berisso, Argentina. The Journal of Infection in Developing Countries 5 (7): 527-534.

Monis, P., R. H. Andrews, G. Mayrhofer, y P. L. Ey. 2003. Genetic diversity within the morphological species Giardia intestinalis and its relationship to host origin. Infection, genetics and evolution 3: 29-38.

Nazeer, J.T., K.E.S. Khalifa, H. von Thien, M.M. El-Sibaei, M.Y. Abdel-amid, R.A.S. Tawfik y E. Tannich. 2013. Use of multiplex real-time PCR for detection of common diarrea causing protozoan parasites in Egypt. Parasitology Research. 112: 595-601.

Plutzer, J., J. Ongerth y P. Karanis. 2010. Giardia taxonomy, phylogeny and epidemiology: Facts and open questions. International Journal of Hygiene and Environmental Health 213 (5): 321-333.

Read, C.M., P. Monis y R.C. Thompson. 2004. Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infection, genetics and evolution 4: 125-130.

Rishniw, M., J. Liotta, M. Bellosa, D. Bowman y K.W. Simpson. 2010. Comparison of 4 Giardia diagnostic tests in diagnosis of naturally acquired anine chronic subclinical giardiasis. Journal of Veterinary Internal medicine 24 (2): 293-297.

Roxstrom-Lindquist, K., D. Palm, D. Reiner, E. Ringqvist y S.G. Svard. 2006. Giardia immunity--an update. Trends in Parasitology 22 (1): 26-31.

Traub, R. J., T. Inpankaew, S.A. Reid, C. Sutthikornchai, Y. Sukthana, I.D. Robertson y R.C. Thompson. 2009. Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok--a critical evaluation of its prevalence using three diagnostic tests in the field n the absence of a gold standard. Acta Tropica Journal 111 (2): 125-132.

Troeger, H., H.J. Epple, T. Schneider, U. Wahnschaffe, R. Ullrich, G.D. Burchard, T. Jelinek, M. Zeitz, M. Fromm y J.D. Schulzke. 2007. Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum. Gut 56 (3): 328-335.

Verweij, J. J., J. Schinkel, D. Laeijendecker, M.A. van Rooyen, L. van Lieshout y A.M. Polderman. 2003. Real-time PCR for the detection of Giardia lamblia. Molecular and Cell Probes 17 (5): 223-225.

Wielinga, C., U. Ryan, R.C. Thompson y P. Monis. 2011. Multi-locus analysis of Giardia duodenalis intra-Assemblage B substitution patterns in cloned culture isolates suggests sub-Assemblage B analyses will require multi-locus genotyping with conserved and variable genes. International Journal for parasitology 41: 495-503.

Downloads

Download data is not yet available.