Un método rápido y efectivo para preparar hormigas para microscopía electrónica de barrido

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Publicado: dic. 31, 2022
Palabras clave:
técnicas de secado al aire, Formicidae, análisis morfológico, tetrametilsilano, SEM

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Roberto J. Guerrero
Grupo de investigación en Insectos Neotropicales, Facultad de Ciencias Básicas, Universidad del Magdalena, Santa Marta, Colombia
https://orcid.org/0000-0003-3244-2754

Resumen

Independientemente de la aplicación de técnicas de microscopía electrónica de barrido (MEB), la preparación adecuada del material biológico a analizar es de suma importancia. En la mayoría de los estudios de hormigas que involucran SEM, las hormigas han sido desecadas por la técnica de punto crítico (CPD por sus siglas en inglés), pero la mayoría de las especies de Dolichoderinae tienen tegumentos delgados y, por lo tanto, tienden a colapsar fácilmente. Para evaluar las ventajas potenciales de un nuevo método sobre la técnica CPD, estas hormigas de tegumentos delgados se trataron con tetrametilsilano (TMS) y luego se secaron al aire. Los resultados obtenidos en este estudio se presentan en microfotografías de barrido. Aquí, detallo un protocolo estandarizado para la preparación de hormigas con TMS antes de SEM. La técnica TMS permite el análisis de casi cinco a seis veces más hormigas que CPD y es más rápido, más fácil, más eficiente y económico que el método CPD.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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Guerrero, R. J. (2022). Un método rápido y efectivo para preparar hormigas para microscopía electrónica de barrido. Intropica, 242–250. Recuperado a partir de https://revistas.unimagdalena.edu.co/index.php/intropica/article/view/4743
Número
Volumen 17 (2), julio-diciembre de 2022
Sección
Artículo de investigación científica y tecnológica
Creative Commons License

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.

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